Compositions and methods for stimulating collagen synthesis in the skin

ABSTRACT

Cosmetic compositions comprising substituted carbamoyl heterocyclic analogs and methods of using such compositions to impart anti-aging benefits to the skin are disclosed. The substituted carbamoyl heterocyclic analogs are believed to have modulatory activity against one or more biochemical pathways implicated in skin aging.

FIELD OF INVENTION

The present invention relates generally to compositions and methods for topical application to the skin which comprise substituted carbamoyl heterocyclic analogs and the use of such compositions to improve the aesthetic appearance of the skin.

BACKGROUND OF THE INVENTION

Collagen is the body's major structural protein and is composed of three protein chains wound together in a tight triple helix. This unique structure gives collagen a greater tensile strength than steel. Approximately 33 percent of the protein in the body is collagen. This protein supports tissues and organs and connects these structures to bones. In tact, bones are also composed of collagen combined with certain minerals such as calcium and phosphorus. Collagen plays a key role in providing the structural scaffolding surrounding cells that helps to support cell shape and differentiation, similar to how steel rods reinforce a concrete block. The mesh-like collagen network binds cells together and provides the supportive framework or environment in which cells develop and function, and tissues and bones heal.

Collagen is created by fibroblasts, which are specialized skin cells located in the dermis. Fibroblasts also produce other skin structural proteins such as elastin (a protein which gives the skin its ability to snap back) and glucosaminoglycans (GAGs). GAGs make up the ground substance that keeps the dermis hydrated. In order to signal or turn on the production of skin structural proteins, fibroblast cells have specially shaped receptors on their outside membranes that act as binding sites to which signal molecules with a matching shape can fit. When the receptors are bound by the correct combination of signal molecules (called fibroblast growth factors, or FGFs), the fibroblast begins the production of collagen. The stimulation of collagen gives the skin its strength, durability, and smooth, plump appearance.

It is therefore an object of the invention to provide new compositions and methods for stimulating collagen. It is a further object of the invention to improve the overall appearance of skin, including treating, reversing, and/or preventing signs of aging, such as skin wrinkles, by stimulating collagen, with cosmetic compositions comprising effective amounts of substituted carbamoyl heterocyclic analogs.

The foregoing discussion is presented solely to provide a better understanding of nature of the problems confronting the art and should not be construed in any way as an admission as to prior art nor should the citation of any reference herein be construed as an admission that such reference constitutes “prior art” to the instant application.

SUMMARY OF TILE INVENTION

In accordance with the foregoing objectives and others, it has surprisingly been found that substituted carbamoyl heterocyclic analogs are stimulators of collagen, and thus are beneficial agents against various signs of intrinsic aging and photo-aging of skin.

In one aspect of the invention, a method is provided for improving the aesthetic appearance of human skin comprising topically applying to an area of the skin in need thereof an effective amount of a substituted carbamoyl heterocyclic analog or a cosmetically acceptable salt thereof in a cosmetically acceptable vehicle.

In another aspect of the invention, cosmetic compositions are provided for improving the aesthetic appearance of skin comprising, in a cosmetically acceptable vehicle, an effective amount of a substituted carbamoyl heterocyclic analog having the structure of formula I:

where ε₁-ε₃ are independently selected from CR_(a), N, or NR^(b), and wherein the ring A is optionally aromatic or may comprise zero, one, or two double bonds;

with the proviso that at least one of ε₁, ε₂, or ε₃ is NR^(b); and in the case where ε₃ is NR^(b), then ε₂ is a nitrogen atom, and the bond between ε₁ and ε₂ is a double bond; in the case where ε₂ is NR^(b), then ε₁ is a nitrogen atom, and the bond between ε₁ and the carbon atom to which R₁ is attached is a double bond; and in the case where ε₁ is NR^(b), then ε₂ is a nitrogen atom, and the bond between ε₂ and ε₃ is a double bond;

R^(b) is an aryl group of the form,

R₁ is selected from a group consisting of H, or a C₁₋₂₀ hydrocarbons, independently selected from alkyl, cycloalkyl, haloalkyl, alkoxyalkyl, alkenyl, alkynyl, alkylaryl, heteroalkyl, heteroarylalkyl, arylalkyl, aryl, aminoalkyl, aminoalkylheteroaryl, alkyloxyaryl, alkyloxyheteroaryl, or heteroaryl group optionally substituted with one or more C₁₋₂₀ alkyl, alkenyl, alkynyl, aryl, or R_(a) groups;

R₂ and R₃ are independently selected from the group consisting of C₁₋₆ alkyl, alkenyl, alkynyl, and aryl, which could be optionally connected to form together with the nitrogen atom to which they are attached a 5, 6, or 7 membered ring, saturated or unsaturated, which optionally further comprises one or more heteroatoms in the ring, selected from oxygen, nitrogen, or sulfur, and wherein R₂ and R₃ are optionally substituted with one or more groups R_(a);

R₄-R₈ are independently selected from the group consisting of R_(a), C₁₋₆ alkyl, alkenyl, alkynyl, and aryl, each of which could be optionally further substituted with one or more groups R_(a);

wherein R_(a) is independently, at each occurrence, selected from hydrogen, halogen (F, Cl, Br, or I); —OH; —NH₂; —NR^(N)R^(N); —SH; —CN; oxo; —CHO; —CO₂H; —O—(C═O)—H; —O—(C═O)—C₁₋₁₀ alkyl; —O—(C═O)—Ar; —(C═O)—O—C₁₋₁₀ alkyl; —(C═O)—O—Ar; —(C═O)—NR^(N)R^(N); —O—C₁₋₁₀ alkyl; —O—Ar; —S—C₁₋₁₀ alkyl; —S—Ar; —Ar; C₁₋₁₀ alkyl; —NR^(N)—CHO; —NR^(N)—(C═O)—C₁₋₁₀ alkyl; —C₁₋₁₀ alkyl—O—C₁₋₁₀ alkyl; perfluoroalkyl; epoxy; azido; thiocyanate; —SO₂—R^(N); or nitro;

wherein R^(N) independently selected, at each occurrence, from hydrogen or a C₁₋₁₆ hydrocarbon radical, optionally substituted with a group R_(a); and where any two adjacent groups R^(N) may together form a 5, 6, or 7 membered ring;

and wherein any two adjacent R groups can be taken together to form a 5-7 member ring, saturated or unsaturated, optionally incorporating O, S, N heteroatoms or substituted with alkyl, haloalkyl, or alkoxy groups;

or a cosmetically acceptable salt thereof.

Also provided is a method of treating one or more signs of skin aging comprising topically applying to skin in need thereof a collagen-stimulating substituted carbamoyl heterocyclic analog according to formula I or a cosmetically acceptable salt thereof.

In another aspect of the invention, a method of treating; reducing, and/or preventing fine lines and/or wrinkles, sagging in human skin, loss of elasticity and molting is provided, comprising topically applying to skin in need thereof, including applying directly to a wrinkle or fine line, a composition comprising a collagen-stimulating substituted carbamoyl heterocyclic analog according to formula I or a cosmetically acceptable salt thereof.

These and other aspects of the present invention will be better understood by reference to the following detailed description.

DETAILED DESCRIPTION

All terms used herein are intended to have their ordinary meaning unless otherwise provided. By “cosmetically acceptable” is meant that a particular component is generally regarding as safe and non-toxic at the levels employed. The term “prevent,” as used herein, includes delaying the onset or progression of a particular sign of skin aging. The term “thin skin” includes skin that becomes thinner with chronological aging as well as prematurely thinned skin, which may be caused, for example, by photo-aging. The phrase “individual in need thereof” refers to a human that could benefit from improved dermal appearance or health, including males or females. The term “skin” includes, without limitation, the lips, skin of the face, hands, arms, neck, legs, in particular thighs, and chest. As used herein, the term “consisting essentially of” is intended to limit the invention to the specified materials or steps and those that do not materially affect the basic and novel characteristics of the claimed invention, as understood from a reading of this specification.

The present invention provides compositions for topical application which comprise an effective amount of substituted carbamoyl heterocyclic analogs or a related compound to treat, reverse, ameliorate and/or prevent signs of skin aging. Such signs of skin aging include without limitation, the following:

-   -   (a) treatment, reduction, and/or prevention of fine lines or         wrinkles,     -   (b) reduction of skin pore size,     -   (c) improvement in skin thickness, plumpness, and/or tautness;     -   (d) improvement in skin suppleness and/or softness;     -   (e) improvement in skin tone, radiance, and/or clarity;     -   (f) improvement in procollagen, collagen production, and/or         elastin production;     -   (g) improvement in maintenance and remodeling of collagen and/or         elastin;     -   (h) improvement in skin texture and/or promotion of         retexturization;     -   (i) improvement in skin barrier repair and/or function;     -   (j) improvement in appearance of skin contours;     -   (k) restoration of skin luster and/or brightness;     -   (l) replenishment of essential nutrients and/or constituents in         the skin;     -   (m) decreased by aging and/or menopause;     -   (n) improvement in skin moisturization;     -   (o) increase in skin elasticity and/or resiliency;     -   (p) treatment, reduction, and/or prevention of skin sagging;     -   (q) reduction of pigment spots;     -   (s) improving the appearance of acne scars or marks;     -   (t) improving the appearance of stretch marks; and/or     -   (u) improvement in the appearance of cellulite.

In practice, the compositions of the invention are applied to skin in need of treatment. That is, skin which suffers from a deficiency or loss in any of the foregoing attributes or which would otherwise benefit from improvement in any of the foregoing skin attributes.

In certain preferred embodiments the compositions and methods of the invention are directed to the prevention, treatment, and/or amelioration of fine lines and/or wrinkles in the skin. In this case, the compositions are applied to skin in need of treatment, by which is meant skin having wrinkles and/or fine lines. Preferably, the compositions are applied directly to the fine lines and/or wrinkles. The compositions and methods are suitable for treating fine lines and/or wrinkles on any surface of the skin, including without limitation, the skin of the face, neck, and/or hands.

The cosmetic compositions for treating a skin condition associated with loss of collagen and/or elastin fiber comprise, in a cosmetically acceptable vehicle, an amount of a substituted carbamoyl heterocyclic analogs effective to enhance collagen. These collagen enhancing/stimulating agents may have the structure of formula (I):

where ε₁-ε₃ are independently selected from CR_(a), N, or NR^(b), and wherein the ring A is optionally aromatic or may comprise zero, one, or two double bonds;

with the proviso that at least one of ε₁, ε₂, or ε₃ is NR^(b); and in the case where ε₃ is NR^(b), then ε₂ is a nitrogen atom, and the bond between ε₁ and ε₂ is a double bond; in the case where ε₂ is NR^(b), then ε₁ is a nitrogen atom, and the bond between ε₁ and the carbon atom to which R₁ is attached is a double bond; and in the case where ε₁ is NR^(b), then ε₂ is a nitrogen atom, and the bond between ε₂ and ε₃ is a double bond;

R^(b) is an aryl group of the form,

R₁ is selected from a group consisting of H, or a C₁₋₂₀ hydrocarbons, independently selected from alkyl, cycloalkyl, haloalkyl, alkoxyalkyl, alkenyl, alkynyl, alkylaryl, heteroalkyl, heteroarylalkyl, arylalkyl, aryl, aminoalkyl, aminoalkylheteroaryl, alkyloxyaryl, alkyloxyheteroaryl, or heteroaryl group optionally substituted with one or more C₁₋₂₀ alkyl, alkenyl, alkynyl, aryl, or R_(a) groups;

R₂ and R₃ are independently selected from the group consisting of C₁₋₆ alkyl, alkenyl, alkynyl, and aryl, which could be optionally connected to form together with the nitrogen atom to which they are attached a 5, 6, or 7 membered ring, saturated or unsaturated, which optionally further comprises one or more heteroatoms in the ring, selected from oxygen, nitrogen, or sulfur, and wherein R₂ and R₃ are optionally substituted with one or more groups R_(a);

R₄-R₃ are independently selected from the group consisting of R_(a), C₁₋₆ alkyl, alkenyl, alkynyl, and aryl, each of which could be optionally further substituted with one or more groups R_(a);

wherein R_(a) is independently, at each occurrence, selected from hydrogen, halogen (F, Cl, Br, or I); —OH; —NH₂; —NR^(N)R^(N); —SH; —CN; oxo; —CHO; —CO₂H; —O—(C═O)—H; —O—(C═O)—C₁₋₁₀ alkyl; —O—(C═O)—Ar; —(C═O)—O—C₁₋₁₀ alkyl; —(C═O)—O—Ar; —(C═O)—NR^(N)R^(N); —O—C₁₋₁₀ alkyl; —O—Ar; —S—C₁₋₁₀ alkyl; —S—Ar; —Ar; —C₁₋₁₀ alkyl; —NR^(N)—CHO; —NR^(N)—(C═O)—C₁₋₁₀ alkyl; —C₁₋₁₀ alkyl-O—C₁₋₁₀ alkyl; perfluoroalkyl; epoxy; azido; thiocyanate; —SO₂—R^(N); or nitro;

wherein R^(N) is independently selected, at each occurrence, from hydrogen or a C₁₋₁₆ hydrocarbon radical, optionally substituted with a group R_(a); and where any two adjacent groups R^(N) may together form a 5, 6, or 7 membered ring;

and wherein any two adjacent R groups can be taken together to form a 5-7 member ring, saturated or unsaturated, optionally incorporating O, S, N heteroatoms or substituted with alkyl, haloalkyl, or alkoxy groups;

and wherein any two adjacent R groups can be taken together to form a 5-7 member ring, saturated or unsaturated, optionally incorporating O, S, and/or N heteroatoms or substituted with alkyl, haloalkyl, or alkoxy groups;

and wherein any R group may be optionally substituted with one or more substituents selected independently at each occurrence from hydrogen; —F; —Cl; —Br; —I; —OH, —OR*; —NH₂; —NHR*; —N(R*)₂; —N(R*)₃ ⁺; —N(R*)—OH; —N(→O)(R*)₂; —O—N(R*)₂; —N(R*)—O—R*; —N(R*)—N(R*)₂; —C═N—R*; —N═C(R*)₂; —C═N—N(R*)₂; —C(═NR*)—N(R*)₂; —SH; —SR*; —CN; —NC; —CHO; —CO₂H; —CO₂ ⁻; —CO₂R*; —(C═O)—S—R*; —O—(C═O)—H; —O—(C═O)—R*; —S—(C═O)—R*; —(C═O)—NH₂; —(C═O)—N(R*)₂; —(C═O)—NHNH₂; —O—(C═O)—NHNH₂; —(C═S)—NH₂; —(C═S)—N(R*)₂; —N(R*)—CHO; —N(R*)—(C═O); —N(R*)—(C═O)—R*; —(C═NR)—O—R*; —O—(C═NR*)—R*, —SCN; —NCS; —NSO; —SSR*; —N(R*)—C(═O)—N(R*)₂; —N(R*)—C(═S)—N(R*)₂; —SO₂—R*; —O—S(═O)₂—R*; —S(═O)₂—OR*; —N(R*)—SO₂—R*; —SO₂—N(R*)₂; —O—SO₃ ⁻; —O—S(═O)₂OR*; —O—S(═O)—OR*; —O—S(═O)—R*; —S(═O)—OR*; S(═O)—R*; —NO; —NO₂; —NO₃; —O—NO; —O—NO₂; —N₃; —N₂—R*; —N(C₂H₄); —Si(—R*)₃; —CF; —O—CF₃; —(C═O)—R*; —PR*₂; —O—P(═O)(OR*)₂; —P(═O)(OR*)₂; ═O; ═S; ═NR*; an aliphatic C₁-C₂₀ hydrocarbon radical; a C₁-C₂₀ aromatic hydrocarbon radical; or a C₁-C₂₀ heteroaryl radical; where R* is independently at each occurrence hydrogen or a saturated, partially saturated, or aromatic C₁-C₂₀ hydrocarbon radical (preferably a C₁-C₆ hydrocarbon, or a C₁-C₃ hydrocarbon) or halogenated derivative thereof;

or a cosmetically acceptable salt thereof

Any nitrogen atom may be optionally oxidized to the N-oxide or can be quarternized, for example with lower alkyl halides, such as methyl, ethyl, propyl, and butyl chloride, bromides, and iodides; dialkyl sulfites like dimethyl, diethyl, dibutyl, and diamyl sulfates, long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides, aralkyl halides such as benzyl and phenethyl bromides, to name a few.

In one embodiment according to formula (I), ε₁ is preferably a nitrogen atom, ε₂ is a group NR^(b) when R^(b) is a substituted aryl ring, and the collagen-stimulating compound is a substituted carbamoyl heterocyclic analog having the structure shown in formula (Ia):

where, R₁, and R₄-R₈, R_(a), and R^(N) are as defined as above;

Q is a 3-7 membered heterocyclic ring, optionally aromatic, and which may comprise zero, one, or two double bonds;

X is selected from CR_(a), C(R_(a))₂, N, O, S, NR^(N), or a bond (i.e. absent); and

L₁ and L₂, are independently selected from a bond or a group X₁-(L₃)_(l)-X₂, where “l” is an integer from 0 to 4; where L₃ is selected from CR_(a), C(R_(a))₂, or a bond; and X₁ and X₂ are independently selected from N, N^(RN), O, S, or a bond; or a cosmetically acceptable salt thereof.

In one embodiment according to formula (Ia), L₁ is —(CH₂)_(m)—, and L₂ is —(CH₂)_(n)—, wherein “m” and “n” are independently selected from integers from 1-3; and X is selected from N, NR^(N), S, O, or a bond; or a cosmetically acceptable salt thereof.

In another embodiment according to formula (Ia), the Q is a heterocycle selected from the group consisting of aziridine, diaziridine, oxaziridine, azetidine, diazetidine, oxazetidine, pyrrolidine, oxazolidine, thiazolidine, imidazolidine, pyrazolidine, pyrrole, imidazole, pyrazole, 1,3,4-triazole, 1,2,3-triazole, piperidine, morpholine; piperazine; and thiomorpholine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

In a preferred embodiment according to formula (Ia), Q is a heterocycle selected from the group consisting of pyrrolidine, morpholino, and piperazine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

In another embodiment according to formula (I), ε₂ is preferably a nitrogen atom, ε₃ is a group NR^(b) when R^(b) a substituted aryl ring, and the collagen-stimulating is compound is a substituted carbamoyl heterocyclic analog having the structure shown in formula (Ib):

where, R₁, and R₄-R₈, R_(a), and R^(N) are as defined above;

Q is a 3-7 membered heterocyclic ring, optionally aromatic, and which may comprise zero, one, or two double bonds;

X is selected from CR_(a), C(R_(a))₂, N, O, S, NR^(N), or a bond (i.e. absent); and

L₁ and L₂ are independently selected from a bond or a group X₁-(L₃)_(l)-X₂, where “l” is an integer from 0 to 4; where L₃ is selected from CR_(a), C(R_(a))₂, or a bond; and X₁ and X₂ are independently selected from N, NR^(N), O, S, or a bond; or a cosmetically acceptable salt thereof.

In one embodiment according to formula (Ib), L₁ is —(CH₂)_(m)—, and L₂ is —(CH₂)_(n)—, wherein “m” and “n” are independently selected from integers from 1-3; and X is selected from N, NR^(N), S, O, or a bond; or a cosmetically acceptable salt thereof.

In another embodiment according to formula (Ib), the Q is a heterocycle selected from the group consisting of aziridine, diaziridine, oxaziridine, azetidine, diazetidine, oxazetidine, pyrrolidine, oxazolidine, thiazolidine, imidazolidine, pyrazolidine, pyrrole, imidazole, pyrazole, 1,3,4-triazole, 1,2,3-triazole, piperidine, morpholine, piperazine, and thiomorpholine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

In a preferred embodiment according to formula (Ib), Q is a heterocycle selected from the group consisting of pyrrolidine, morpholino, and piperazine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

In another embodiment according to formula (I), ε₁ and ε₂ are preferably nitrogen atoms, ε₃ is a group NR^(b) when R^(b) is a substituted aryl ring, and the collagen-stimulating compound is a substituted carbamoyl heterocyclic analog having the structure shown in formula (Ic):

where, R₁, and R₄-R₈, R_(a), and R^(N) are as defined as above;

Q is a 3-7 membered heterocyclic ring, optionally aromatic, and which may comprise zero, one, or two double bonds;

X is selected from CR_(a), C(R_(a))₂, N, O, S, NR^(N), or a bond (i.e. absent); and

L₁ and L₂ are independently selected from a bond or a group X₁-(L₃)_(l)-X₂, where “l” is an integer from 0 to 4; where L₃ is selected from CR_(a), C(R_(a))₂, or a bond; and X₁ and X₂ are independently selected from N, NR^(N), O, S, or a bond; or a cosmetically acceptable salt thereof.

In one embodiment according to formula (Ic), L₁ is —(CH₂)_(m)—, and L₂ is —(CH₂)_(n)—, wherein “m” and “n” are independently selected from integers from 1-3; and X is selected from N, NR^(N), S, O, or a bond; or a cosmetically acceptable salt thereof.

In another embodiment according to formula (Ic), the Q is a heterocycle selected from the group consisting of aziridine, diaziridine, oxaziridine, azetidine, diazetidine, oxazetidine, pyrrolidine, oxazolidine, thiazolidine, imidazolidine, pyrazolidine, pyrrole, imidazole, pyrazole, 1,3,4-triazole, 1,2,3-triazole, piperidine, morpholine, piperazine, and thiomorpholine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

In a preferred embodiment according to formula (Ic), Q is a heterocycle selected from the group consisting of pyrrolidine, morpholino, and piperazine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

In another embodiment according to formula (I), ε₂ and ε₃ are preferably nitrogen atoms, ε₁ is a group NR^(b) when R^(b) is a substituted aryl ring, and the collagen-stimulating compound is a substituted carbamoyl heterocyclic analog having the structure shown in formula (Id):

where, R₁, and R₄-R₈, R_(a), and R^(N) are as defined as above;

Q is a 3-7 membered heterocyclic ring, optionally aromatic, and which may comprise zero, one, or two double bonds;

X is selected from CR_(a), C(R_(a))₂, N, O, S, NR^(N), or a bond (i.e. absent); and

L₁ and L₂ are independently selected from a bond or a group X₁-(L₃)_(l)-X₂, where “l” is an integer from 0 to 4; where L₃ is selected from CR_(a), C(R_(a))₂, or a bond; and X₁ and X₂ are independently selected from N, NR^(N), O, S, or a bond; or a cosmetically acceptable salt thereof.

In one embodiment according to formula (Id), L₁ is —(CH₂)_(m)—, and L₂ is —(CH₂)_(n)—, wherein “m” and “n” are independently selected from integers from 1-3; and X is selected from N, NR^(N), S, O, or a bond; or a cosmetically acceptable salt thereof.

In another embodiment according to formula (Id), the Q is a heterocycle selected from the group consisting of aziridine, diaziridine, oxaziridine, azetidine, diazetidine, oxazetidine, pyrrolidine, oxazolidine, thiazolidine, imidazolidine, pyrazolidine, pyrrole, imidazole, pyrazole, 1,3,4-triazole, piperidine, morpholine, piperazine, and thiomorpholine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

In a preferred embodiment according to formula (Id), Q is a heterocycle selected from the group consisting of pyrrolidine, morpholino, and piperazine, each being optionally substituted with one or more groups R_(a), or a cosmetically acceptable salt thereof.

Other illustrative compounds according to the invention are provided below.

The invention embraces the use of cosmetically or pharmaceutically acceptable (e.g., non-toxic and/or non-irritating) salts. Examples of the salts of the compounds in the present invention include salts with alkali metals such as sodium and potassium; salts with alkaline-earth metals such as calcium and magnesium; salts with amines such as monoethanolamine; salts with inorganic acids such as hydrochloric acid and sulfuric acid; and salts with organic acids such as citric acid and acetic acid. Special mention may be made of hydrochloride salts.

The cosmetic compositions according to the invention can be formulated in a variety of forms for topical application and will comprise from about 0.0001% to about 1% by weight of one or more compounds according to formula (I), and preferably will comprise from about 0.001% to about 0.5% by weight, and more preferably from about 0.01% to about 0.2% by weight. The compositions will comprise an effective amount of the substituted carbamoyl heterocyclic analog compounds according to formula (I), by which is meant an amount sufficient to enhance collagen and/or stimulate collagen in a given area of skin when topically applied thereto.

The composition may be formulated in a variety of product forms, such as, for example, a lotion, cream, serum, spray, aerosol, cake, ointment, essence, gel, paste, patch, pencil, towelette, mask, stick, foam, elixir, concentrate, and the like, particularly for topical administration. Preferably the composition is formulated as a lotion, cream, ointment, or gel.

The compositions can include a cosmetically acceptable vehicle. Such vehicles may take the form of any known in the art suitable for application to skin and may include water; vegetable oils; mineral oils; esters such as octal palmitate, isopropyl myristate and isopropyl palmitate; ethers such as dicapryl ether and dimethyl isosorbide; alcohols such as ethanol and isopropanol; fatty alcohols such as cetyl alcohol, cetearyl alcohol, stearyl alcohol and biphenyl alcohol; isoparaffins such as isooctane, isododecane and is hexadecane; silicone oils such as cyclomethicone, dimethicone, dimethicone cross-polymer, polysiloxanes and their derivatives, preferably organomodified derivatives; hydrocarbon oils such as mineral oil, petrolatum, isoeicosane and polyisobutene; polyols such as propylene glycol, glycerin, butylene pentylene glycol and hexylene glycol; waxes such as beeswax and botanical waxes; or any combinations or mixtures of the foregoing.

The vehicle may comprise an aqueous phase, an oil phase, an alcohol, a silicone phase or mixtures thereof. The cosmetically acceptable vehicle may also comprise an emulsion. Non-limiting examples of suitable emulsions include water-in-oil emulsions, oil-in-water emulsions, silicone-in-water emulsions, water-in-silicone emulsions, wax-in-water emulsions, water-oil-water triple emulsions or the like having the appearance of a cream, gel or microemulsions. The emulsion may include an emulsifier, such as a nonionic, anionic or amphoteric surfactant.

The oil phase of the emulsion preferably has one or more organic compounds, including emollients; humectants (such as propylene glycol and glycerin); other water-dispersible or water-soluble components including thickeners such as veegum or hydroxyalkyl cellulose; gelling agents, such as high MW polyacrylic acid, i.e. CARBOPOL 934; and mixtures thereof. The emulsion may have one or more emulsifiers capable of emulsifying the various components present in the composition.

The compounds suitable for use in the oil phase include without limitation, vegetable oils; esters such as octyl palmitate, isopropyl myristate and isopropyl palmitate; ethers such as dicapryl ether; fatty alcohols such as cetyl alcohol, stearyl alcohol and behenyl alcohol; isoparaffins such as isooctane, isododecane and isohexadecane; silicone oils such as dimethicones, cyclic silicones, and polysiloxanes; hydrocarbon oils such as mineral oil, petrolatum, isoeicosane and polyisobutene; natural or synthetic waxes; and the like. Suitable hydrophobic hydrocarbon oils may be saturated or unsaturated, have an aliphatic character and be straight or branched chained or contain alicyclic or aromatic rings. The oil-containing phase may be composed of a singular oil or mixtures of different oils.

Hydrocarbon oils include those having 6-20 carbon atoms, more preferably 10-16 carbon atoms. Representative hydrocarbons include decane, dodecane, tetradecane, tridecane, and C₈₋₂₀ isoparaffins. Paraffinic hydrocarbons are available from Exxon under the ISOPARS trademark, and from the Permethyl Corporation. In addition, C₈₋₂₀ paraffinic hydrocarbons such as C12 isoparaffin (isododecane) manufactured by the Permethyl Corporation having the tradename Permethyl 99ATM are also contemplated to be suitable, Various commercially available C₁₆ isoparaffins, such as isohexadecane (having the trademark. Permethyl™) are also suitable. Examples of preferred volatile hydrocarbons include polydecanes such as isododecane and isodecane, including for example, Permethyl-99A (Presperse Inc.) and the C₇-C₈ through C₁₂-C₁₅ isoparaffins such as the Isopar Series available from Exxon Chemicals. A representative hydrocarbon solvent is isododecane.

The oil phase may comprise one or more waxes, including for example, rice bran wax, carnauba wax, ouricurry wax, candelilla wax, montan waxes, sugar cane waxes, ozokerite, polyethylene waxes, Fischer-Tropsch waxes, beeswax, microcrystalline wax, silicone waxes, fluorinated waxes, and any combination thereof.

Non-limiting emulsifiers included emulsifying waxes, emulsifying polyhydric alcohols, polyether polyols, polyethers, mono- or di-ester of polyols, ethylene glycol mono-stearates, glycerin mono-stearates, glycerin di-stearates, silicone-containing emulsifiers, soya sterols, fatty alcohols such as cetyl alcohol, fatty acids such as stearic acid, fatty acid salts, and mixtures thereof. The preferred emulsifiers include soya sterol, cetyl alcohol stearic acid, emulsifying wax, and mixtures thereof. Other specific emulsifiers that can be used in the composition of the present invention include, but are not limited to, one or more of the following: sorbitan esters; polyglyceryl-3-diisostearate; sorbitan monostearate, sorbitan tristearate, sorbitan sesquioleate, sorbitan monooleate; glycerol esters such as glycerol monostearate and glycerol monooleate; polyoxyethylene phenols such as polyoxyethylene octyl phenol and polyoxyethylene nonyl phenol; polyoxyethylene ethers such as polyoxyethylene cetyl ether and polyoxyethylene stearyl ether; polyoxyethylene glycol esters; polyoxyethylene sorbitan esters; dimethicone copolyols; polyglyceryl esters such as polyglyceryl-3-diisostearate; glyceryl laurate; Steareth-2, Steareth-10, and Steareth-20, to name a few. Additional emulsifiers are provided in the INCI Ingredient Dictionary and Handbook 11th Edition 2006, the disclosure of which is hereby incorporated by reference.

These emulsifiers typically will be present in the composition in an amount from about 0.001% to about 10% by weight, in particular in an amount from about 0.01% to about 5% by weight, and more preferably, below 1% by weight.

The oil phase may comprise one or more volatile and/or non-volatile silicone oils. Volatile silicones include cyclic and linear volatile dimethylsitoxane silicones. In one embodiment, the volatile silicones may include cyclodimethicones, including tetramer (D4), pentamer (D5), and hexamer (D6) cyclomethicones, or mixtures thereof. Particular mention may be made of the volatile cyclomethicone-hexamethyl cyclotrisiloxane, octamethyl-cyclotetrasiloxane, and decamethyl-cyclopentasiloxane. Suitable dimethicones are available from Dow Corning under the name Dow Corning 200® Fluid and have viscosities ranging from 0.65 to 600,000 centistokes or higher. Suitable non-polar, volatile liquid silicone oils are disclosed in U.S. Pat. No. 4,781,917, herein incorporated by reference in its entirety. Additional volatile silicones materials are described in Todd et al., “Volatile Silicone Fluids for Cosmetics”, Cosmetics and Toiletries, 91:27-32 (1976), herein incorporated by reference in its entirety. Linear volatile silicones generally have a viscosity of less than about 5 centistokes at 25° C., whereas the cyclic silicones have viscosities of less than about 10 centistokes at 25° C. Examples of volatile silicones of varying viscosities include Dow Corning 200, Dow Corning 244, Dow Corning 245, Dow Corning 344, and Dow Corning 345, (Dow Corning Corp.); SF-1204 and SF-1202 Silicone Fluids (G.E. Silicones), GE 7207 and 7158 (General Electric Co.); and SWS-03314 (SWS Silicones Corp.). Linear, volatile silicones include low molecular weight polydimethylsiloxane compounds such as hexamethyldisiloxane, octamethyltrisiloxane, decamethyltetrasilaxane, and dodecamethylpentasilaxane, to name a few.

Non-volatile silicone oils will typically comprise polyalkylsiloxanes, polyarylsiloxanes, polyalkylarylsiloxanes, or mixtures thereof. Polydimethylsitoxanes are preferred non-volatile silicone oils. The non-volatile silicone oils will typically have a viscosity from about 10 to about 60,000 centistokes at 25° C., preferably between about 10 and about 10,000 centistokes, and more preferred still between about 10 and about 500 centistokes; and a boiling point greater than 250° C. at atmospheric pressure. Non limiting examples include dimethyl polysiloxane (dimethicone), phenyl trimethicone, and diphenyldimethicone. The volatile and non-volatile silicone oils may optionally be substituted will various functional groups such as alkyl, aryl, amine groups, vinyl, hydroxyl, haloalkyl groups, alkylaryl groups, and acrylate groups, to name a few.

The water-in-silicone emulsion may be emulsified with a nonionic surfactant (emulsifier) such as, for example, polydiorganosiloxane-polyoxyalkylene block copolymers, including those described in U.S. Pat. No. 4,122,029, the disclosure of which is hereby incorporated by reference. These emulsifiers generally comprise a polydiorganosiloxane backbone, typically polydimethylsitoxane, having side chains comprising -(EO)m- and/or -(PO)n- groups, where EO is ethyleneoxy and PO is 1,2-propyleneoxy, the side chains being typically capped or terminated with hydrogen or lower alkyl groups (e.g., C₁₋₆, typically C₁₋₃). Other suitable water-in-silicone emulsifiers are disclosed in U.S. Pat. No. 6,685,952, the disclosure of which is hereby incorporated by reference herein. Commercially available water-in-silicone emulsifiers include those available from Dow Corning under the trade designations 3225C and 5225C Formulation Aid; Silicone SF-1528 available from General Electric; ABIL EM 90 and EM 97, available from Goldschmidt Chemical Corporation (Hopewell, Va.); and the SILWET series of emulsifiers sold by OSI Specialties (Danbury, Conn.).

Examples of water-in-silicone emulsifiers include, but are not limited to, dimethicone PEG 10/15 crosspolymer, dimethicone copolyol, cetyl dimethicone copolyol, PEG-15 lauryl dimethicone crosspolymer, laurylmethicone crosspolymer, cyclomethicone and dimethicone copolyol, dimethicone copolyol (and) caprylic/capric triglycerides, polyglyceryl-4 isostearate (and) cetyl dimethicone copolyol (and) hexyl laurate, and dimethicone copolyol (and) cyclopentasiloxane. Preferred examples of water-in-silicone emulsifiers include, without limitation, PEG/PPG-18/18 dimethicone (trade name 5225C, Dow Corning), PEG/PPG-19/19 dimethicone (trade name BY25-337, Dow Corning), Cetyl PEG/PPG-10/1 dimethicone (trade name Abil EM-90, Goldschmidt Chemical Corporation), PEG-12 dimethicone (trade name SF 1288, General Electric), lauryl PEG/PPG-18/18 methicone (trade name 5200 FORMULATION AID, Dow Corning), PEG-12 dimethicone crosspolymer (trade name 9010 and 9011 silicone elastomer blend, Dow Corning), PEG-10 dimethicone crosspolymer (trade name KSG-20. Shin-Etsu), and dimethicone PEG-10/15 crosspolymer (trade name KSG-210, Shin-Etsu).

The water-in-silicone emulsifiers typically will be present in the composition in an amount from about 0.001% to about 10% by weight, in particular in an amount from about 0.01% to about 5% by weight, and more preferably, below 1% by weight.

The aqueous phase of the emulsion may include one or more additional solvents, including lower alcohols, such as ethanol, isopropanol, and the like. The volatile solvent may also be a cosmetically acceptable ester such as butyl acetate or ethyl acetate; ketones such as acetone or ethyl methyl ketone; or the like.

The oil-containing phase will typically comprise from about 10% to about 99%, preferably from about 20% to about 85%, and more preferably from about 30% to about 70% by weight, based on the total weight of the emulsion, and the aqueous phase will typically comprise from about 1% to about 90%, preferably from about 5% to about 70%, and more preferably from about 20% to about 60% by weight of the total emulsion. The aqueous phase will typically comprise from about 25% to about 100%, more typically from about 50% to about 95% by weight water.

The compositions may include liposomes. The liposomes may comprise other additives or substances and/or may be modified to more specifically reach or remain at a site following administration.

The composition may optionally comprise other cosmetic actives and excipients, obvious to those skilled in the art including, but not limited to, fillers, emulsifying agents, antioxidants, surfactants, film formers, chelating agents, gelling agents, thickeners, emollients, humectants, moisturizers, vitamins, minerals, viscosity and/or rheology modifiers, sunscreens, keratolytics, depigmenting agents, retinoids, hormonal compounds, alpha-hydroxy acids, alpha-keto acids, anti-mycobacterial agents, antifungal agents, antimicrobials, antivirals, analgesics, lipidic compounds, anti-allergenic agents, H1 or H2 antihistamines, anti-inflammatory agents, anti-irritants, antineoplastics, immune system boosting agents, immune system suppressing agents, anti-acne agents, anesthetics, antiseptics, insect repellents, skin cooling compounds, skin protectants, skin penetration enhancers, exfollients, lubricants, fragrances, colorants, depigmenting agents, hypopigmenting agents, preservatives, stabilizers, pharmaceutical agents, photostabilizing agents, sunscreens, and mixtures thereof. In addition to the foregoing, the cosmetic compositions of the invention may contain any other compound for the treatment of skin disorders.

Colorants may include, for example, organic and inorganic pigments and pearlescent agents. Suitable inorganic pigments include, but are not limited to, titanium oxide, zirconium oxide and cerium oxide, as well as zinc oxide, iron oxide, chromium oxide and ferric blue. Suitable organic pigments include barium, strontium, calcium, and aluminium lakes and carbon black. Suitable pearlescent agents include mica coated with titanium oxide, with iron oxide, or with natural pigment.

Various fillers and additional components may be added. Fillers are normally present in an amount of about 0 weight % to about 20 weight %, based on the total weight of the composition, preferably about 0.1 weight % to about 10 weight %. Suitable fillers include without limitation silica, treated silica, talc, zinc stearate, mica, kaolin, Nylon powders such as Orgasol™, polyethylene powder, Teflon™, starch, boron nitride, copolymer microspheres such as Expancel™ (Nobel Industries), Polytrap™ (Dow Corning) and silicone resin microbeads (Tospearl™ from Toshiba), and the like.

In one embodiment of the invention, the compositions may include additional skin actives such as, but are not limited to, botanicals, keratolytic agents, desquamating agents, keratinocyte proliferation enhancers, collagenase inhibitors, elastase inhibitors, depigmenting agents, anti-inflammatory agents, steroids, anti-acne agents, antioxidants, salicylic acid or salicylates, thiodipropionic acid or esters thereof, and advanced glycation end-product (AGE) inhibitors.

In a specific embodiment, the composition may comprise at least one additional botanical, such as, for example, a botanical extract, an essential oil, or the plant itself. Suitable botanicals include, without limitation, extracts from Abies pindrow, Acacia catechu, Anogeissus latifolia, Asmunda japonica, Azadirachta indica, Butea frondosa, Butea monosperma, Cedrus deodara, Entblica officinalis, Ficus benghalensis, Glycyrrhiza glabra, Ilex purpurea Hassk, Inniula racemosa, Ligusticum chiangxiong, Ligusticum lucidum, Mallotus philippinensis, Mimusops elengi, Morinda citrifolia, Moringa oleifera, Naringi crenulata, Nerium indicum, Psoralea corylifolia, Stenoloma chusana, Terminalia bellerica, tomato glycolipid, Sapindus rarak, Humulus japonicus, Eclipta prostrate, Amorphophallus campanulatus, Sesbania grandiflora, Pouzolzia pentandra, Melicope hayesii, Ixora chinensis, Erythina indica, Medemia noblis, Tiliacora triandra, Derris scandens, Portulaca oleracea, Alisma orientale, and mixtures thereof.

The composition may comprise additional active ingredients having anti-aging benefits, as it is contemplated that synergistic improvements may be obtained with such combinations. Exemplary anti-aging components include, without limitation, botanicals (e.g., Butea Frondosa extract); thiodipropionic acid (TDPA) and esters thereof; retinoids (e.g., all-trans retinoic acid, 9-cis retinoic acid, phytanic acid and others); hydroxy acids (including alpha-hydroxyacids and beta-hydroxyacids), salicylic acid and salicylates; exfoliating agents (e.g., glycolic acid, 3,6,9-trioxaundecanedioic acid, etc.), estrogen synthetase stimulating compounds (e.g., caffeine and derivatives); compounds capable of inhibiting 5 alpha-reductase activity (e.g., linolenic acid, linoleic acid, finasteride, and mixtures thereof); barrier function enhancing agents (e.g., ceramides, glycerides, cholesterol and its esters, alpha-hydroxy and omega-hydroxy fatty acids and esters thereof, etc.); collagenase inhibitors; and elastase inhibitors; to name a few. Further additional actives useful for topical application to skin include perilla oil, mangostine, palmitoyl lysytaminovaleroyl lysine (palmitoyl K-ava-K), palmatoyl tetrapeptide-10 (KTFK), L-4-thiazolylatanine, cis-6-nonenol, desthiobiotin, N-(4-mesyloxybenzyl)-N-methoxyethyl-4-chlorobenzene carboxamide, N-(4-mesyloxybenzyl)-N-isobutyl benzenesulfonamide, retinyl oleate, carvacrol, and mixtures thereof.

Exemplary retinoids include, without limitation, retinoic acid (e.g., all-trans or 13-cis) and derivatives thereof, retinol (Vitamin A) and esters thereof, such as retinol palmitate, retinol acetate and retinol propionate, and salts thereof.

In another embodiment, the topical compositions of the present invention may also include one or more of the following: a skin penetration enhancer, an emollient, a skin plumper, an optical diffuser, a sunscreen, an exfoliating agent, and an antioxidant.

An emollient provides the functional benefits of enhancing skin smoothness and reducing the appearance of fine lines and coarse wrinkles Examples include isopropyl myristate, petrolatum, isopropyl lanolate, silicones (e.g., methicone, dimethicone), oils, mineral oils, fatty acid esters, or any mixtures thereof. The emollient may be preferably present from about 0.1 wt % to about 50 wt % of the total weight of the composition.

A skin plumper serves as a collagen enhancer to the skin. An example of a suitable, and preferred, skin plumper is palmitoyl oligopeptide. Other skin plumpers are collagen and/or other glycosaminoglycan (GAG) enhancing agents. When present, the skin plumper may comprise from about 0.1 wt % to about 20 wt % of the total weight of the composition.

An optical diffuser is a particle that changes the surface optometrics of skin, resulting in a visual blurring and softening of, for example, lines and wrinkles. Examples of optical diffusers that can be used in the present invention include, but are not limited to, boron nitride, mica, nylon, polymethylmethacrylate (PMMA), polyurethane powder, sericite, silica, silicone powder, talc, Teflon, titanium dioxide, zinc oxide, or any mixtures thereof. When present, the optical diffuser may be present from about 0.01 wt % to about 20 wt % of the total weight of the composition.

A sunscreen for protecting the skin from damaging ultraviolet rays may also be included. Preferred sunscreens are those with a broad range of UVB and UVA protection, such as octocrylene, avobenzone (Parsol 1789), octyl methoxycinnamate, octyl salicylate, oxybenzone, homosylate, benzophenone, camphor derivatives, zinc oxide, and titanium dioxide, When present, the sunscreen may comprise from about 0.01 wt % to about 70 wt % of the composition.

Suitable exfoliating agents include, for example, alpha-hydroxyacids, beta-hydroxyacids, oxaacids, oxadiacids, and their derivatives such as esters, anhydrides and salts thereof. Suitable hydroxy acids include, for example, glycolic acid, lactic acid, malic acid, tartaric acid, citric acid, 2-hydroxyalkanoic acid, mandelic acid, salicylic acid and derivatives thereof. A preferred exfoliating agent is glycolic acid. When present, the exfoliating agent may comprise from about 0.1 wt % to about 80 wt % of the composition.

An antioxidant functions, among other things, to scavenge free radicals from skin to protect the skin from environmental aggressors. Examples of antioxidants that may be used in the present compositions include compounds having phenolic hydroxy functions, such as ascorbic acid and its derivatives/esters; beta-carotene; catechins; curcumin; ferulic acid derivatives (e.g. ethyl ferulate, sodium ferulate); gallic acid derivatives (e.g., propyl gallate); lycopene; reductic acid; rosmarinic acid; tannic acid; tetrahydrocurcumin; tocopherol and its derivatives; uric acid; or any mixtures thereof. Other suitable antioxidants are those that have one or more thiol functions (—SH), in either reduced or non-reduced form, such as glutathione, lipoic acid, thioglycolic acid, and other sulfhydryl compounds. The antioxidant may be inorganic, such as bisulfites, metabisulfites, sulfites, or other inorganic salts and acids containing sulfur. Compositions of the present invention may comprise an antioxidant preferably from about 0.001 wt % to about 10 wt %, and more preferably from about 0.01 wt % to about 5 wt %, of the total weight of the composition.

Other conventional additives include: vitamins, such as tocopherol and ascorbic acid; vitamin derivatives such as ascorbyl monopalmitate; thickeners such as hydroxyalkyl cellulose; gelling agents; structuring agents such as bentonite, smectite, magnesium aluminum silicate and lithium magnesium silicate; metal chelating agents such as EDTA; pigments such as zinc oxide and titanium dioxide; colorants; emollients; and humectants.

It is preferred that the composition be essentially free of components having a strong oxidizing potential, including for example, organic or inorganic peroxides. By “essentially free of” these components is meant that the amounts present are insufficient to have a measurable impact on the collagen I, desmogleins, and/or dermatopontin enhancing activity of the substituted carbamoyl heterocyclic analog. In some embodiments, this will be, on a molar basis in relation to the amount of substituted carbamoyl heterocyclic analog, less than 1%.

In one embodiment, the composition of the invention comprising a substituted carbamoyl heterocyclic analog may have a pH between about 1 and about 8. In certain embodiments, the pH of the composition will be acidic, i.e., less than 7.0, and preferably will be between about 2 and about 7, more preferably between about 3.5 and about 5.5.

The invention provides a method for treating aging skin by topically applying a composition comprising a substituted carbamoyl heterocyclic analog, preferably in a cosmetically acceptable vehicle, over the affected area for a period of time sufficient to reduce, ameliorate, reverse or prevent dermatological signs of aging. This method is particularly useful for treating signs of skin photoaging and intrinsic aging.

Generally, the improvement in the condition and/or aesthetic appearance is selected from the group consisting of: reducing dermatological signs of chronological aging, photo-aging, hormonal aging, and/or actinic aging; preventing and/or reducing the appearance of lines and/or wrinkles; reducing the noticeability of facial lines and wrinkles, facial wrinkles on the cheeks, forehead, perpendicular wrinkles between the eyes, horizontal wrinkles above the eyes, and around the mouth, marionette lines, and particularly deep wrinkles or creases; preventing, reducing, and/or diminishing the appearance and/or depth of lines and/or wrinkles; improving the appearance of suborbital lines and/or periorbital lines; reducing the appearance of crow's feet; rejuvenating and/or revitalizing skin, particularly aging skin; reducing skin fragility; preventing and/or reversing of loss of glycosaminoglycans and/or collagen, in particular collagen I; ameliorating the effects of estrogen imbalance; preventing skin atrophy; preventing, reducing, and/or treating hyperpigmentation; minimizing skin discoloration; improving skin tone, radiance, clarity and/or tautness; preventing, reducing, and/or ameliorating, skin sagging; improving skin firmness, plumpness, suppleness and/or softness; improving procollagen and/or collagen production; improving skin texture and/or promoting retexturization; improving skin barrier repair and/or function; improving the appearance of skin contours; restoring skin luster and/or brightness; minimizing dermatological signs of fatigue and/or stress; resisting environmental stress; replenishing ingredients in the skin decreased by aging and/or menopause; improving communication among skin cells; increasing cell proliferation and/or multiplication; increasing skin cell metabolism decreased by aging and/or menopause; retarding cellular aging; improving skin moisturization; enhancing skin thickness; increasing skin elasticity and/or resiliency; enhancing exfoliation; improving microcirculation; decreasing and/or preventing cellulite formation; and any combinations thereof.

Without wishing to be bound by any particular theory, it is believed that the compositions of the present invention enhance and improve the aesthetic appearance of skin by stimulation of collagen.

The composition will typically be applied to the skin one, two, or three times daily for as long as is necessary to achieve desired anti-aging results. The treatment regiment may comprise daily application for at least one week, at least two weeks, at least four weeks, at least eight weeks, or at least twelve weeks. Chronic treatment regimens are also contemplated.

The substituted carbamoyl heterocyclic analog active component is topically applied to an “individual in need thereof,” by which is meant an individual that stands to benefits from reducing visible signs of skin damage or aging. In a specific embodiment, the substituted carbamoyl heterocyclic analog component is provided in a pharmaceutically, physiologically, cosmetically, and dermatologically-acceptable vehicle, diluent, or carrier, where the composition is topically applied to an affected area of skin and left to remain on the affected area in an amount effective for improving the condition and aesthetic appearance of skin.

In one embodiment, methods for treating fine lines and wrinkles comprise topically applying the inventive substituted carbamoyl heterocyclic analog compositions to the skin of an individual in need thereof, e.g., topically application directly to the fine line and/or wrinkle in an amount and for a time sufficient to reduce the severity of the fine lines and/or wrinkles or to prevent or inhibit the formation of new fine lines and/or wrinkles. The effect of a composition on the formation or appearance of fine lines and wrinkles can be evaluated qualitatively, e.g., by visual inspection, or quantitatively, e.g., by microscopic or computer assisted measurements of wrinkle morphology (e.g., the number, depth, length, area, volume and/or width of wrinkles per unit area of skin). This embodiment includes treatment of wrinkles on the skin of the hands, arms, legs, neck, chest, and face, including the forehead,

It is also contemplated that the compositions of the invention will be useful for treating thin skin by topically applying the composition to thin skin of an individual in need thereof. “Thin skin” is intended to include skin that is thinned due to chronological aging, menopause, or photo-damage. In some embodiments, the treatment is for thin skin in men, whereas other embodiments treat thin skin in women, pre-menopausal or post-menopausal, as it is believed that skin thins differently with age in men and women, and in particular in women at different stages of life.

The method of the invention may be employed prophylactically to forestall aging including in patients that have not manifested signs of skin aging, most commonly in individuals under 25 years of age. The method may also reverse or treat signs of aging once manifested as is common in patients over 25 years of age.

The following examples are meant to demonstrate certain aspects of the invention in a non-limiting fashion.

EXAMPLES Example 1

The compounds 1-5, and 8-9 were assayed for stimulation of collagen synthesis as follows. Human dermal fibroblasts (Cascade Biologics, Portland, Oreg.) were plated at 10,000 cells/well in 96-well culture plates in supplemented medium (DMEM, 10% Fetal Bovine Serum, 1% Penicillin/Streptomycin and 1% L-Glutamine) overnight in humidified atmosphere of 10% CO₂ at 37° C. The next day, the medium was replaced with fresh medium (DMEM, 1% Penicillin/Streptomycin and 1% L-Glutamine) and the compounds dissolved DMSO were added to the wells in triplicate at a concentration of 0.0005%, DMSO solution was used a control. Following 48-hour incubation, the plates were removed from the incubator and the medium from each well was collected for the procollagen assay.

Collagen production was measured using procollagen type I C-peptide (PIP) EIA kit (Takara Bio, Inc., Japan). Briefly, the conditioned medium was diluted 1:10 in Sample Diluent, 20 μl of diluted conditioned medium and 100 μl of antibody-POD conjugate solution were added to the wells of the Takara HASA plate. The ELISA plate was incubated at 37° C. for 3 hours before the wells were washed four times with 400 μl of 1×PBS. At the end of wash, 100 μl of substrate solution (supplied with kit) was added to the wells and incubated at room temperature for 15 minutes. The reaction was stopped by adding 100 μl of 1N sulfuric acid to the wells. The absorbance was measured on a spectrophotometer at 450 nm wavelength. The amount of procollagen peptide in the conditioned medium was calculated from the standard curve. The stimulation of collagen production was shown as an increase in collagen over the control.

For compounds 1-5 the results were:

TABLE 1 Compound R^(b) R_(a) R₁

% Collagen Stimulation 1

H

73.2% 2

23.1% 3

H

13.5% 4

H

12.5% 5

H

45.23% 

For compounds 8-9 the results were:

TABLE 2 Compound R^(b) R_(a) R₁

% Collagen Stimulation 8

H

83.7% 9

H

62.2%

Results illustrate that the compounds included in Tables 1 and 2 are collagen stimulators, and that compounds 1, 8 and 9 are potent stimulators of collagen synthesis.

Example 2

Other illustrative examples are shown below in Tables 3 and 4.

TABLE 3 Compound R^(b) R_(a) R₁

6

H

7

TABLE 4 Compound R^(b) R_(a) R₁

10

H

11

H

12

H

13

H

Example 3 Illustrative Compositions

The cosmetic compositions set forth in Table 5 are illustrative of the invention and are useful for topical application to the skin to enhance its aesthetic appearance.

TABLE 5 Composition 1 2 3 4 5 Components Weight % Compound 1 of Table 1 0.01 0.05 — — — Compound 5 of Table 1 — — 0.1 — — Compound 8 of Table 2 — — — 0.05 — Compound 9 of Table 2 0.05 Acrylates/C10-30 Alkyl 1 1 1 1 1 Acrylate Crosspolymer Cetyl Ethylhexanoate 10 10 10 10 10 C12-15 Alkyl Benzoate 3.9 3.9 3.9 3.9 3.9 Isopropyl Isostearate 3 3 3 3 3 Diisopropyl dimer 0.1 0.1 0.1 0.1 0.1 dillinoleate Tocopheryl acetate 0.5 0.5 0.5 0.5 0.5 Butylene glycol 2 2 2 2 2 Propylene glycol 1 1 1 1 1 Dimethicone PEG-7 0.5 0.5 0.5 0.5 0.5 isostearate Methyl gluceth-20 0.5 0.5 0.5 0.5 0.5 Triethanolamine 1 1 1 1 1 Acrylates/acrylamide 1.5 1.5 1.5 1.5 1.5 copolymer/mineral oil DMDM Hydantoin/ 0.4 0.4 0.4 0.4 0.4 Iodopropynylbutylcarbonate Deionized water q.s. q.s. q.s. q.s. q.s. Total: 100 100 100 100 100

All references including patent applications and publications cited herein are incorporated herein by reference in their entirety and for all purposes to the same extent as if each individual publication or patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety for all purposes. Many modifications and variations of this invention can be made without departing from its spirit and scope, as will be apparent to those skilled in the art. The specific embodiments described herein are offered by way of example only, and the invention is to be limited only by the terms of the appended claims, along with the full scope of equivalents to which such claims are entitled. 

The invention claimed is:
 1. A method of stimulating collagen synthesis in human skin comprising topically applying to an area of the skin in need thereof, in a cosmetically acceptable vehicle, an effective amount of a collagen-stimulating compound having the formula I:

where: R₁ is selected from the group consisting of H, or a C₁₋₆ alkyl, alkenyl, alkynyl, aryl, or heteroaryl group optionally substituted with one or more C₁₋₆ alkyl, alkenyl, alkynyl, aryl, or R_(a) groups; R₄-R₈ are independently selected from the group consisting of R_(a), C₁₋₆ alkyl, alkenyl, alkynyl, and aryl, each of which could be optionally further substituted with one or more groups R_(a); wherein R_(a) is independently, at each occurrence, selected from hydrogen, F, Cl, Br, I; —OH; —NH₂; —NR^(N)R^(N); —SH; —CN; oxo; —CHO; —CO₂H; —O—(C═O)—H; —O—(C═O)—C₁₋₁₀ alkyl; —O—(C═O)—Ar; —(C═O)—O—C₁₋₁₀ alkyl; —(C═O)—O—Ar; —(C═O)—NR^(N)R^(N); —O—C₁₋₁₀ alkyl; —O—Ar; —S—C₁₋₁₀ alkyl; —S—Ar; —Ar; —C₁₋₁₀ alkyl; —NR^(N)—CHO; —NR^(N)—(C═O)—C₁₋₁₀ alkyl; —C₁₋₁₀ alkyl—O—C₁₋₁₀ alkyl; perfluoroalkyl; epoxy; azido; thiocyanate; —SO₂—R^(N); or nitro; wherein R^(N) is independently selected, at each occurrence, from hydrogen or a C₁₋₁₆ hydrocarbon radical, optionally substituted with a group R_(a); and where any two adjacent groups R^(N) may together form a 5, 6, or 7 membered ring; or a cosmetically acceptable salt thereof.
 2. The method according to claim 1 wherein R₄ to R₈ are independently hydrogen, methyl, ethyl, propyl, chloro, or bromo, with at least two of R₄ to R₈ being hydrogen.
 3. The method according to claim 1, wherein R_(a) is hydrogen, methyl, ethyl, propyl, and R₁ is hydrogen, methyl, ethyl, or propyl.
 4. The method according to claim 1, wherein R_(a) is methyl, ethyl, propyl, and R₁ is hydrogen, methyl, ethyl, or propyl.
 5. The method according to claim 1, wherein said collagen-stimulating compound of formula I or a cosmetically acceptable salt thereof is applied to said skin at least once daily for a period of at least four weeks.
 6. The method according to claim 1, wherein the compound has the structure:

and wherein R^(b), R_(a), R₁, and Q are as set forth below: R^(b) R_(a) R₁

H

H

H 